How is an angle head centrifuge set up?

How is an angle head centrifuge set up?

• A. Tubes remain vertical at all times
• B. Tube slots are set between 10 and 60 degrees
• C. Tubes are arranged in a circular pattern
• D. The rotor spins at a fixed speed without tilt

Answer: (B)

In an angle head centrifuge, the tubes are held in slots that are tilted relative to the rotor axis. This tilt is the defining feature, with the slots set at an angle typically between about 10 and 60 degrees depending on the rotor design. That angled position causes the centrifugal force to act along the length of the tube, promoting clear separation of components and causing the sediment to collect along the lower inner wall of the tube. It also makes decanting easier because the supernatant remains above the sediment along the angled axis. The exact angle is chosen to suit the sample type and rotor construction. Descriptions that imply tubes are simply vertical or that only a circular arrangement is used don’t capture this essential tilt of the angle head.

In an angle head centrifuge, the tubes are held in slots that are tilted relative to the rotor axis. This tilt is the defining feature, with the slots set at an angle typically between about 10 and 60 degrees depending on the rotor design. That angled position causes the centrifugal force to act along the length of the tube, promoting clear separation of components and causing the sediment to collect along the lower inner wall of the tube. It also makes decanting easier because the supernatant remains above the sediment along the angled axis. The exact angle is chosen to suit the sample type and rotor construction. Descriptions that imply tubes are simply vertical or that only a circular arrangement is used don’t capture this essential tilt of the angle head.